All publications

Construction of spheroids from melanocytes of different ethnic origins: screening model and variation

  • Poster

53rd Annual ESDR Meeting (4-7 September 2024, Lisbon, Portugal) - Sep 2024

Nicolas Bechetoille, Sébastien Bonnet et Chloé Lorion


Melanogenesis is a complex porcess, ans its in vitro modeling remains a challenge to develop relevant test suitable for rapid preclinical evaluation of the efficacy of lightening products to modulate human skin pigmentation.
Melanocytes are known to lose their ability to synthesize melanin in monolayer culture, even though the melanin content correlates with the ethnic background of the donors. Tissue engineering is an alternative to 2D culture because the cell behaviors in 3D microenvironment are closer to skin physiology.
We therefore compared the efficacy of 3 assays as high-throughput in vitro systems for skin-lightening drug screening: (i) Monolayer 2D culture of proliferative melanocytes, (ii) Microtissue 3D spheroid of non-proliferative melanocytes, (iii) Epidermal tissue 3D model containing functional melanocytes.

Elastogenic potential and antisagging properties of a novel Murraya koenigii extract

  • Scientific publication

Journal of Cosmetic Dermatology - 2023

C. Lorion PhD, V. Bardin, S. Bonnet, A. Lopez-Gaydon, B. Vogelgesang, N. Bechetoille PhD

The newly discovered extract of Murraya koenigii leafy stems represents an innovative antiaging ingredient suited for elasticity-boosting and antisagging claims.

A multiparametric, stepwise in vitro approach to identify anti-dark circle and anti-puffiness ingredients

  • Poster

IFSCC Congress - 2022

C. Lorion PhD, C. Lavastre, A. Rascalou, A. Lopez-Gaydon, S. Bonnet, T. Rinaldi, V. Charton, B. Vogelgesang, N. Bechetoille PhD

A unique plant extract with promising anti-puffiness and anti-dark circle potential has been identified using the stepwise selection model.

Advanced translational cosmetics: using the world’s first non-invasive bioimpedance 3D bioprinted skin chips to link cosmetics lab testing to humans

  • Poster

IFSCC Congress - 2022

McGuckin, Bechetoille, Legues, Milet, Besseyre, Boyer, Ferrier, Sanchez, Forraz, Vogelgesang

This model enables advanced translational lab-to-donor data to help bring sophisticated cosmetics ingredients to market faster, safely and more affordably.

A NaDES extract of Rose ‘Jardin de Granville®’ displays pro-resolving and epidermal strengthening properties

  • Poster

IFSCC Congress - 2022

Pecher V., Franchi J., Charton V., Dubourdeau M, Van Goethem E., Baillif V., Nizard C., Choisy P., Vogelgesang B., Bechetoille N.

The fructose/propanediol/water (NaDES FPW113) extract of “Jardin de Granville” Rose flowers has demonstrated great potential in vitro and ex vivo to support epidermal homeostasis and counteract the inflammaging process.

Unexpected elasticity-booting properties of a traditional ayurvedic plant

  • Poster

ESDR - 2021

Chloé Lorion PhD, Sébastien Bonnet, Amandine Lopez-Gaydon, Anna Drillat, Virginie Charton, Nicolas Bechetoille PhD

Murraya koenigii extract displays undeniable elasticity boosting properties, highlighted at molecular, cellular
and tissue levels, and appears as a powerful anti-aging ingredient.

Scrutiny of the supramolecular structure of bio-sourced fructose/glycerol/water ternary mixtures: towards green low transition temperature mixtures

  • Scientific publication

Journal of Molecular Liquids - 2021

B. Caprin, V. Charton, J-D. Rodier, B. Vogelgesang, A. Charlot, F. Da Cruz-Boisson, E. Fleury

This comprehensive study is a prerequisite for a further industrial implementation of eco-designed solvents in various applicative attractive domains, as in cosmetic field.

Biobased and non-ionic LTTMs composed of fructose and glycerol: water and temperature impact on the supramolecular organization

  • Oral communication

International Meeting on Deep Eutectic Systems - 2021

Benoit Caprin

The objective is to u nderstand interactions at the molecular level for various F/G/W mixtures and establish a relationship between their micro and macroscopical features.

Generation of homogeneous, scaffold-free adipose spheroids for screening of lipolytic agents

  • Poster

ESDR - 2021

Sébastien Bonnet, Amandine Lopez-Gaydon, Cindy Lavastre, Chloé Lorion, Nicolas Bechetoille

Adipose spheroids provide an interesting in vitro model of pre-adipocyte maturation and adipocyte expansion.